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In this work, a real-time label-free microwave diagnostic approach using Co-Planar Waveguide (CPW) design has been demonstrated for glucose detection. This mechanism has tremendous potential for the biomedical applications. Here, glucose biosensor is implemented with 50Ω CPW transmission line, where the centre localized 3mm diameter of CPW transmission line has been used for the sensing. Glucose sensor is implemented utilizing low cost multilayer PCB and polymer Poly-Di-Methyl-Siloxane(PDMS)doi:10.13164/re.2019.0491 fatcat:b6wtfmx5jjh37nanyffaj3fo2i