Nuclear localization signal domain of HDAC3 is necessary and sufficient for the expression regulation of MDR1

Hyunmi Park, Youngmi Kim, Deokbum Park, Dooil Jeoung
2014 BMB Reports  
Histone acetylation/deacetylation has been known to be associated with the transcriptional regulation of various genes. The role of histone deacetylase-3 in the expression regulation of MDR1 was investigated. The expression level of HDAC3 showed an inverse relationship with the expression level of MDR1. Wild-type HDAC3, but not catalytic mutant HDAC3 S424A , negatively regulated the expression of MDR1. Wild-type HDAC3, but not catalytic mutant HDAC3 S424A , showed binding to the promoter
more » ... es of HDAC3. HDAC3 regulated the expression level, and the binding of Ac-H3 K9/14 and Ac-H4 K16 around the MDR1 promoter sequences. The nuclear localization signal domain of HDAC3 was necessary, and sufficient for the binding of HDAC3 to the MDR1 promoter sequences and for conferring sensitivity to microtubule-targeting drugs. [BMB Reports 2014; 47(6): 342-347]
doi:10.5483/bmbrep.2014.47.6.169 pmid:24286324 pmcid:PMC4163870 fatcat:4wsso3gtcfb3lnhoqbsei6npiy