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A Simple Plate Method for the Screening and Detection of Fucoidanases
2015
Achievements in the Life Sciences
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A novel method was developed for the screening and detection of fucoidanase activity in bacterial colonies using fucoidan-agarose plates and staining with hexadecyltrimethylammonium bromide (Cetavlon). Colonies with fucoidanase activity were indicated by a transparent halo. The medium containing undegraded fucoidan showed as a milk-colored background, but areas with degraded fucoidan under the colonies were visualized as a transparent halo.
doi:10.1016/j.als.2015.12.002
fatcat:wlrwdswchbaizdtz642tpascoa