Purpose: To analyze the antimicrobial properties of silicon oil (Óleo de Silicone ® , Oph thalmos, Brazil) on in vitro bacterial growth of different microorganisms related to endophthalmitis. Methods: The following microorganisms were analyzed: (1) Pseudomonas aerugi no sa (ATCC 27583); (2) Escherichia coli (ATCC 25922); (3) Staphylococcus aureus (ATCC 25923); (4) Staphylococcus epidermidis (ATCC 12228); (5) Candida albicans (ATCC 10231); (6) Klebsiella pneumoniae (ATCC 13883); and (7)

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... ); and (7) Streptococcus pneumoniae (ATCC 49619). The plates were incubated at 35 ± 2 o C and its growth examined after 24 hours. An empty disk was placed in the center of each plate as a control. Results: No inhibition halos were verified in any of the plates containing the four different concentrations of the bacterial inocula. Conclusions: The silicon oil 1000 cps does not have any effect on bacterial growth of any of the studied microrganisms.