Microbial fuel cells (MFC) have gained focus due to their diversity in operating conditions & substrates for the generation of sustainable green energy. In the present study, novel MFC has been fabricated using the recyclable aluminum can as air-cathode and graphite rod as anode for the treatment of domestic wastewater and simultaneous power generation. Three different substrate (COD) concentrations, high (>800 mg.L-1), medium (250 mg.L-1 - 800 mg.L-1) and low (<250 mg.L-1) were used. The

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... m COD removal efficiencies, voltage generation, power densities were found to be 80%, 0.71 V, and 304.46 mW.m-2 respectively in high strength wastewater setup. In both medium and low strength wastewater setups, after 288 hours, the COD was reduced below 50 mg.L-1 thus limiting the electricity generation substantially. Setup with low-strength wastewater produced a maximum CE (%) of 13.80. Overall results showed that although high-strength wastewater produced better and maximum power densities, medium and low-strength wastewater setups were more consistent in energy generation throughout the experiment.