Activation of Toll-like receptor 9 and production of epitope specific antibody by liposome-encapsulated CpG-DNA

Dong-Bum Kim, Hyung-Joo Kwon, Young-Hee Lee
2011 BMB Reports  
Several investigators have shown that CpG-DNA has outstanding effects as a Th1-responsive adjuvant and that its potent adjuvant effects are enhanced by encapsulation with a liposome of proper composition. In this study, we showed that encapsulation with phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (DOPE): cholesterol hemisuccinate (CHEMS) complex enhances the immunostimulatory activity of CpG DNA and the binding of CpG-DNA to TLR9. We also examined involve- ment of myeloid differentiation
more » ... differentiation protein (MyD88) and NF-κB activation in liposome-encapsulated CpG-DNA-induced IL-8 promoter activation. In this manuscript, the natural phosphodiester bond CpG-DNA encapsulated by DOPE : CHEMS complex is designated as Lipoplex(O). Importantly, we successfully screened B cell epitopes of envelope protein (E protein) of hepatitis C virus (HCV-E) and attachment glycoprotein G of human respiratory syncytial virus (HRSV-G) by immunization with complexes of several peptides and Lipoplex(O) without carriers. Therefore, Lipoplex(O) is potentially applicable as a universal adjuvant for peptide-based epitope screening and antibody production. [BMB reports 2011; 44(9): 607-612] Production of antibody using encapsulated CpG-DNA Dongbum Kim, et al.
doi:10.5483/bmbrep.2011.44.9.607 pmid:21944255 fatcat:cffn4ipib5aizprfcp54vscyc4