Transport and processing of staphylococcal enterotoxin B

R K Tweten, J J Iandolo
1983 Journal of Bacteriology  
A larger, membrane-bound form of staphylococcal enterotoxin B was shown by in vivo pulse-chase analysis to be the kinetic precursor to extracellular enterotoxin B. Processing of the enterotoxin B precursor molecules can apparently occur either cotranslationally or posttranslationally. Subcellular fractionation of cells revealed that all of the precursor toxin was associated with the membrane fraction. Once processed and released from the membrane, it was transiently associated with the cell
more » ... before being released into the extracellular environment. The cell-wall-associated enterotoxin B was completely resistant to protease treatment and to extraction by highor low-salt solutions at 0 to 2°C, although it could be easily released from the cell by removal of the cell wall with lysostaphin. These data imply that newly formed enterotoxin B may be temporarily sequestered in specialized regions that require cell wall integrity before being released into the extracellular environment. ess, including evidence that newly formed, ma-297 on May 9, 2020 by guest
doi:10.1128/jb.153.1.297-303.1983 fatcat:x7ucglhsefemvpptibz7n7hici