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Expression and biochemical characterization of a multifunctional glycosidase from the thermophilic Bacillus licheniformis SR01
2017
Journal of General and Applied Microbiology
A gene (gkdA) (741 bp) encoding a putative protein of 247 amino acids was cloned from the Bacillus licheniformis SR01. The protein was expressed in Escherichia coli BL21 with a molecular mass estimated by SDS-PAGE of approximately 28.03 kDa and showed a calculating isoelectric point (pI) of 6.42. Structure analysis and function identification showed that the enzyme was a multifunctional glycosidase. Its specific activity was 0.013 U/m m m m mg. The recombinant glycosidase showed a maximum
doi:10.2323/jgam.2016.12.001
pmid:28835594
fatcat:idddyf3kcfc3jbwhexkolesnmu