Formulation and Antibacterial Activity of Natural Disinfectant Combination of Psidium guajava and Piper betle Leaf Infusion Against Staphylococcus aureus
Proceedings of the Conference on Health and Wellbeing in Modern Society (CHW 2021)
Spraying disinfectant all over the body in the disinfection room is being used to prevent the spread of the SARS-CoV-2 as the cause of the Covid-19 disease. Due to chemical disinfectants have high toxicity to the body, a relatively safe natural disinfectant is needed. The purposes of this study were to obtain a natural disinfectant solution formula combination of guava leaf infusion (Psidium guajava L.) and betle leaf infusion (Piper betle L.) and to determine the antibacterial activity of
... formulas. In this research, a combination formula of guava leaf and betle leaf infusion with three formulas were formulated, contains 20% guava leaf infusion and 25% betle leaf with ratio of 1: 3, 1: 1, and 3:1 respectively, namely F2, F3 and F4. F0 was chemical disinfectant as positive control and F1 as negative control. Antibacterial activity test against Staphylococcus aureus conducted on a petri dish using disc diffusion method. The highest inhibition zone was formed by F4 with average diameter of 10.2 mm, categorized as strong level of antibacterial activity against Staphylococcus aureus. The results confirmed that natural disinfectant solution formula combination of guava leaf infusion and betle leaf infusion had potential agents as natural disinfectant.