Isolation and Purification of Keratinase from Streptomyces matensis (MPLS-1)

Pavani, Mallika, Prof Girijasankar
2016 Citation: Pavani.M et al. Ijppr.Human   unpublished
A new keratinase extracted from Streptomyces matensis (MPLS-1) was isolated and purified in this study. Solid ammonium sulfate was selected to precipitate the enzyme. Its proper adding mass was also determined. Through solid ammonium sulphate precipitation and liquid chromatography via the DEAE-Sephadex-LH 100 using the feather powder as substrate, 2.4-fold purification with a yield of 26.66% was obtained. The purification effect was determined through SDS-PAGE, and the molecular weight of
more » ... ular weight of enzyme was found at 34kDa. It is simple purification step and high yield using a cheap medium attest to the great biotechnological potential of keratinase, especially in environment protection and in recycling valuable materials from wastes.