Horse gram (Macrotyloma uniflorum Lam.) is a popular pulse, locally known as Gaheth belongs to the family Fabaceae that still remain an under exploited legume. The present study was carried out to investigate the phytochemical profile and analyze the chemical composition of leaves of Macrotyloma uniflorum. The leaves powder was successively extracted with petroleum ether, n-hexane, ethanol, methanol, and ethyl acetate. Phytochemical analysis shows the presence of flavonoids, tannins,

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... saponins, anthocyanins, phenols, proteins, alkaloids and carbohydrates. To isolate and analyze the chemical composition in ethanol crude extracts from leaves of Macrotyloma uniflorum by gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis provided different peaks determining the presence of seven different phytochemical compounds namely hexadecanoic acid, methyl ester (8.81%), 1,2,4-trioxolane-2-octanoic acid, 5-octyl-, methyl ester (8.53%), 10-octadecenoic acid, methyl ester(21.04%), n-hexadecanoic acid (20.59%), oleic acid (22.10%), [1,1'-bicyclopropyl]-2-octanoic acid, 2'-hexyl-, methyl ester (12.64%), and 2-hexadecanol (6.25%).This study helps to predict the formula and structure of biomolecules which can be used as drugs and further investigation may lead to the development of drug formulation.