A Python script to merge Sanger sequences

Cen Chen, Bingguo Lu, Xiaofang Huang, Chuyun Bi, Lili Zhao, Yunzhuo Hu, Xuanyang Chen, Shiqiang Lin, Kai Huang
2021 PeerJ  
Merging Sanger sequences is frequently needed during the gene cloning process. In this study, we provide a Python script that is able to assemble multiple overlapping Sanger sequences. The script utilizes the overlapping regions within the tandem Sanger sequences to merge the Sanger sequences. The results demonstrate that the script can produce the merged sequence from the input Sanger sequences in a single run. The script offers a simple and free method for merging Sanger sequences and is useful for gene cloning.
doi:10.7717/peerj.11354 pmid:33987023 pmcid:PMC8086567 fatcat:hxozygfv5rhwtndpjvbzdccsyi