Application of polyelectrolyte complex (PEC) as a biological material is expected because it does not inhibit cell adhesion or proliferation. In this study, we cultured gingival fibroblasts in a PEC-coated culture dish, and investigated the expression pattern of a cell adhesion molecule, integrin, and that of tyrosine-phosphorylation to analyze intracellular signal transmission to biologically evaluate PEC as a functional biological material for the dental field. The following findings were

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... ined. 1. The integrin expression in cells cultured in PEC-coated dishes was comparable or higher than that in the control group. 2. Integrin and vinculin were present in the cytoplasm of cells cultured in PEC-coated dishes, and their expression patterns were similar to those in the control group. 3. The tyrosine-phosphorylating protein expression in cells cultured in PEC-coated dishes was similar to that in the control group, suggesting that the function of the intracellular signal transmission system was normal in cells cultured on PEC. The above findings revealed that cell culture in PEC-coated dishes did not inhibit integrin expression or tyrosine phosphorylation in the intracellular signal transmission system.