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Na+/K(+)-ATPase activity was demonstrated in the midgut of Anopheles stephensi. More than 80% of the total ATPase activity was sensitive to inhibition by ouabain with an IC50 of 4.5 x 10(-7) +/- 0.3 x 10(-7) mol l-1 and with maximal inhibition occurring at 10(-4) mol l-1. This ouabain-sensitive Na+/K(+)-ATPase was maximally activated at a Mg2+:ATP ratio of 1:1.3, with a Km of 0.3 mmol l-1 and a Vmax of 2.4 mumol Pi mg-1 protein min-1 for ATP. Maximal activation was reached at 15 mmol l-1 K+pmid:8382729 fatcat:jhcub2upnvbh3jayy6ml45wx4u