Polypharmacology-based Approach for Screening of Traditional Chinese Medicinal extract and Multi-dimensional Targeting Verification [post]

Zhiyong Wu, Chunli Xia, Ze Wang, Jiaxin Bao, Rui Li, Muhammad Ishfaq, Jichang Li
2021 unpublished
Background: Natural products and their unique polypharmacology offer significant advantages for finding novel therapeutics particularly for the treatment of complex diseases. Meanwhile, natural products in traditional Chinese medicine possess drug-like properties. In this study, we used the previously established co-infection model of Mycoplasma gallisepticum and Escherichia coli as a representative of complex diseases. The multi-omics joint analysis was used to reverse screen TCMs from the
more » ... ese medicinal database and then targeted verification was conducted from multiple dimensions. Results: The results showed that the Chinese herbal compound screened by the target network played a good therapeutic effect in the case of co-infection. Furthermore, the four methods were performed at gene, protein, and metabolite levels, as well as molecular docking in vitro respectively, which were used to verify the multi-target therapeutic effect. Conclusions: These data suggest that we may provide a new method to validate target combinations of natural products that can be used to optimize their multiple structure-activity relationships to obtain drug-like natural product derivatives. Furthermore, the study could establish a new methodology for the research of Chinese herbal medicinal extract and provide a new multi-target treatment method for the occurrence of co-infection.
doi:10.21203/rs.3.rs-142256/v1 fatcat:cumalszl7zcsrd4qqfwlmuxuei