Steam cell ・ based self −assembled tissue ( scSAr ) bio−syn 血 esized 丘om synovi 一 drived mesenchymal stem cell had a great potential f () r the repair and regeneration ofbiological so 丘 tissues . However, the mechanical prope而 es of the scSAT were insuMcient f {) r clinical applica 価on . A candidate solution to the problem is to promote the gerreration ofthe extracellular matrix in止 e scSAr culturing under cyclic strain . in the present stUdy , an elastio tissue cultUre plate was designed and 亘

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... ocal strain of the plate was investigated . And mesenchymal stem cells were cultured in 血 e presence of ascorbic acid fbr 14days on the elastio plates , then cu 苴 tUred under cyclic 5 ・ 6% strai皿 at 1! 30 H 乞, 7 ・ 9% strain at l18 Hz or Static culture fbr 14days . Results revealed that the each area strain of elastic tissue culture plate was homogeneous until I5% strah1 . And , scSAT cUltUred under large cyclic strain became 血 er and tensile streng 血 was lower . However , tensile strength ofscSAT cultured under small strain tended to be higher. Key Mords : Stem cell based self −assembled tissue ( scSAr ) , Mesenchymal stem cell , Cyclic strain , Elastic plate 1. 緒言