Oxidation of D-sorbitol to L-sorbose with immobilized cells of Gluconobacter suboxydans
固定化Gluconobacter属菌体によるD‐ソルビトールのL‐ソルボースへの酸化

Makiyo IKI, Seiichi HONMA, Ko AIDA
1988 Journal of Home Economics of Japan  
Microbial transformation of D-sorbitol to L-sorbose was studied with immobilized whole cells of bacteria, Gluconobacter suboxydans var. a IFO 3254 which has the strong sorbitol dehydrogenase (SLDH) activity. The resting cells of late logarithmic growth phase were immobilized. The entrapping with Ca-alginate was the most favorable immobilization method. By this method, cells could convert D-sorbitol to L-sorbose constantly through 15th reusing test (20 hr × 15). SLDH of cells could be activated
more » ... y the addition of small amount of nitrogen sources such as yeast extract and peptone.
doi:10.11428/jhej1987.39.197 fatcat:loeivbwenzcdxbcbcw5oulh6b4