Migration of Human Melanoma Cells on Hyaluronate Is Related to CD44 Expression

Luc Thomas, Takafumi Etoh, Ivan Stamenkovic, Martin C. Mihm, H Randolph Byers
1993 Journal of Investigative Dermatology  
Phenotypic and functional aspects of melanomahyaluronate interactions were investigated by studying the expression of CD44, cell migration, .and transmembrane penetration of human melanoma cellimes on hyaluronatecoated substrates. Expression of CD44 was tested by flow cytometry on seven human melanoma cell lines. Strong reactivity with anti-CD44 monoclonal antibody was observed in four of seven of the cell lines. Migration studies of CD44(+) cell lines on hyaluronic acid-and
more » ... chondroitin-6-sulfatecoated substrates, using time-lapse video-microscopy, showed a dramatic dose-dependent increase in migration rate on hyaluronate but not on chondroitin-6-sulfate. Moreover, CD44(-) cell lines showed no modification in migration rate on either substrate. Addition of soluble hyaluronate produced a dose-dependent inhibition of acceleration of CD44(+) cells
doi:10.1111/1523-1747.ep12462776 pmid:8429233 fatcat:ad3qngyuerfhrpe3tk6fmigmlu