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This study aimed to evaluate the fish fauna composition and distribution in different environments, up- and downstream the Gavião Peixoto Dam, in the Jacaré-Guaçu River basin. Most of the sampled ichthyofauna is autochthonous, and the majority of the species belongs to the orders Characiformes and Siluriformes. The order Characiformes comprised most of the specimens caught, Astyanax altiparanae being the most abundant species. All the studied environments were characterized by the massivedoi:10.1590/s1676-06032011000100010 fatcat:nivj55gw5jdrdbrj3bnkdpbzzi