A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange

Yoshihisa Ito, Yusuke Izawa, Toshihisa Osaki, Koki Kamiya, Nobuo Misawa, Satoshi Fujii, Hisatoshi Mimura, Norihisa Miki, Shoji Takeuchi
2020 Micromachines  
Lipid-bilayer devices have been studied for on-site sensors in the fields of diagnosis, food and environmental monitoring, and safety/security inspection. In this paper, we propose a lipid-bilayer-on-a-cup device for serial sample measurements using a pumpless solution exchange procedure. The device consists of a millimeter-scale cylindrical cup with vertical slits which is designed to steadily hold an aqueous solution and exchange the sample by simply fusing and splitting the solution with an
more » ... e solution with an external solution. The slit design was experimentally determined by the capabilities of both the retention and exchange of the solution. Using the optimized slit, a planar lipid bilayer was reconstituted with a nanopore protein at a microaperture allocated to the bottom of the cup, and the device was connected to a portable amplifier. The solution exchangeability was demonstrated by observing the dilution process of a blocker molecule of the nanopore dissolved in the cup. The pumpless solution exchange by the proposed cup-like device presents potential as a lipid-bilayer system for portable sensing applications.
doi:10.3390/mi11121123 pmid:33352964 fatcat:zbsgim5y6nh6hkuusvsrenf54e