Primary Human Immune Response to Neisseria meningitidis Serogroup C in Interleukin-12--Treated Severe Combined Immunodeficient Mice Engrafted with Human Peripheral Blood Lymphocytes

M. A. J. Westerink, D. W. Metzger, W. A. Hutchins, A. R. Adkins, P. F. Holder, L. B. Pais, L. L. Gheesling, G. M. Carlone
1997 Journal of Infectious Diseases  
Lack of primary immune response in severe combined immunodeficient (SCID) mice engrafted with human peripheral blood lymphocytes (hu-PBL) has limited the applicability of this model. Use of human cytokines, in particular interleukin (IL)-12, was studied in the hu-PBL-SCID model. SCID mice were treated with IL-12 and reconstituted with hu-PBL in T replacement factor. The hu-PBL-SCID mice were immunized with serogroup C meningococcal polysaccharide (MCPS). The MCPS-specific antibody response was
more » ... etermined by ELISA. Thirteen of the 15 immunized, IL-12-treated hu-PBL-SCID mice demonstrated a primary human antibody response to MCPS ranging from 0.25 to 3.3 ILg/mL, while no MCPS-specific antibody response was detectable in the 18 controls. Expression of cross-reactive idiotypic markers found on human anti-MCPS antibodies in the immunized hu-PBL-SCID mice was similar to that observed in immunized volunteers.
doi:10.1093/infdis/175.1.84 pmid:8985200 fatcat:uz4kl5f54fch5h2jrr456ghzai