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Background-Studies of cardiac gene transfer rely on postmortem analysis using histologic staining or enzyme assays. Noninvasive imaging of the temporal and spatial characteristics of cardiac gene expression in the same subject offers significant advantages. Methods and Results-Rats underwent direct myocardial injection via left thoracotomy with adenovirus-expressing firefly luciferase (Ad-CMV-Fluc; nϭ30). The reporter substrate D-luciferin was injected intraperitoneally. Serial images weredoi:10.1161/01.cir.0000014984.95520.ad pmid:11940538 fatcat:awuznntfnzgdxacd4xoqqlbeuu