Kinetic analysis of translocation through nuclear pore complexes

Katharina Ribbeck, Dirk Görlich
<span title="2001-03-15">2001</span> <i title="Wiley"> <a target="_blank" rel="noopener" href="" style="color: black;">EMBO Journal</a> </i> &nbsp;
The mechanism of facilitated translocation through nuclear pore complexes (NPCs) is only poorly understood. Here, we present a kinetic analysis of the process using various model substrates. We ®nd that the translocation capacity of NPCs is unexpectedly high, with a single NPC allowing a mass¯ow of nearly 100 MDa/s and rates in the order of 10 3 translocation events per second. Our data further indicate that high af®nity interactions between the translocation substrate and NPC components are
more &raquo; ... pensable for translocation. We propose a'selective phase model' that could explain how NPCs function as a permeability barrier for inert molecules and yet become selectively permeable for nuclear transport receptors and receptor±cargo complexes.
<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="">doi:10.1093/emboj/20.6.1320</a> <a target="_blank" rel="external noopener" href="">pmid:11250898</a> <a target="_blank" rel="external noopener" href="">pmcid:PMC145537</a> <a target="_blank" rel="external noopener" href="">fatcat:wxplbptitvccxbpr6hohlkyhbq</a> </span>
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