Kinetic analysis of translocation through nuclear pore complexes
Katharina Ribbeck, Dirk Görlich
<span title="2001-03-15">2001</span>
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The mechanism of facilitated translocation through nuclear pore complexes (NPCs) is only poorly understood. Here, we present a kinetic analysis of the process using various model substrates. We ®nd that the translocation capacity of NPCs is unexpectedly high, with a single NPC allowing a mass¯ow of nearly 100 MDa/s and rates in the order of 10 3 translocation events per second. Our data further indicate that high af®nity interactions between the translocation substrate and NPC components are
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... pensable for translocation. We propose a'selective phase model' that could explain how NPCs function as a permeability barrier for inert molecules and yet become selectively permeable for nuclear transport receptors and receptor±cargo complexes.
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