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Rapid and Direct Detection of Clostridium chauvoei by PCR of the 16S-23S rDNA Spacer Region and Partial 23S rDNA Sequences
Journal of Veterinary Medical Science
Clostridium chauvoei causes blackleg, which is difficult to distinguish from the causative clostridia of malignant edema. Therefore, a single-step PCR system was developed for specific detection of C. chauvoei DNA using primers derived from the 16S-23S rDNA spacer region and partial 23S rDNA sequences. The specificity of the single-step PCR system was demonstrated by testing 37 strains of clostridia and 3 strains of other genera. A 509 bp PCR product, which is a C. chauvoei-specific PCRdoi:10.1292/jvms.62.1275 pmid:11193343 fatcat:uompjsrj5jd6dglix3gknwmrhm