Some studies on Graminicolours Didymella spp. in New Zealand [article]

Michelle Alexandra Mace, University Of Canterbury
The aim of this thesis was to elucidate aspects of the biology of Didymella species present on Graminicolous hosts in New Zealand. The taxonomy of Didymella spp. and their anamorphs was investigated. Four main types were found and these corresponded to Didymella exitialis D. phleina, D.graminicola and Didymosphaeria loliina. The anamorph states were identified as the Ascochyta state of D. exitialis, Ascochyta phyllachoroides Sacc. and Malb. Forma melicae, Ascochyta state of D. graminicola and
more » ... e Ascochyta state of D. loliina. Two of the isolates identified as the Ascochyta state of D. graminicola could also be classified as A. sorghi. The production of the teleomorph in culture was not achieved on a consistent basis. The teleomorph was produced by four isolates that were plated on CD-V 8 agar. Only pseudothecia and ascii were produced; no ascospores could be found. The teleomorph was produced on one other occasion, when isolates on PDA had been dried down slowly. Conidia of Didymella germinate on leaf surfaces between three and five hours after inoculation at 25°C and 98% RH. Production of appressoria occurs approximately 30 hours after inoculation at 20°C and 98% RH. The method of penetration of the leaf surface is direct with no preferred sites for penetration. A subcuticular, intramural mycelium then develops between the cuticle and the epidermis until the host becomes stressed, or the leaf material starts to senesce. Symptoms affecting less than one percent of the leaf area developed on inoculated plants, no increase in the rate of leaf senescence of infected plants compared to uninoculated plants was observed. Didymella spp. are frequently isolated from symptomless surface sterilised leaf material during the wheat-growing season. Didymella spp. are initially isolated after the first leaf unfolds and can be isolated sporadically from other leaves throughout the growing season, until the leaves senesce. During the 1995/96 growing season the application of azoxystrobin significantly reduced the levels of Didym [...]
doi:10.26021/8478 fatcat:z3nrnb4debb6nhgo6jjzo3evie