Induktions-und Nachweismethoden für Stilbene bei Vitaceen Methods for induction and determination of stilbenes in Vitaceae

U Stein, G Hoos
1984 Vitis   unpublished
S u m m a r y .-The stilbenes resveratrol and E-viniferin produced by leaves of different grapevine species and varieties in response to certain stimulants (Botrytis infection, UV radiation, sugar solutions, mucic acid) have been identified by use of quantitative thin-layer chromatography. Without artificial induction, only small concentrations of resveratrol and/or E-viniferin could be measured in the leaves of some grapevines. Leaf pieces were placed on filter cardboard soaked with an aqueous
more » ... ked with an aqueous solution of mucic acid. The edges of the pieces excreted stilbenes into the cardboard, the concentrations of which were estimated under long wavelength (366 nm) UV radiation. A significant correlation was shown between the data obtained by visual appraisal and the results from thin-layer chromatography. Hence, this visual method allows quick and sufficiently reliable classification of grapevines according to their stilbene production. The susceptibility to B. cinerea was negatively correlated with the ability of grape leaves to produce stilbenes after defined stimulation. The grapevines investigated could be classified as low, middle or highly resistant. A few exceptions showed low stilbene production together with low susceptibility to B. cinerea. The stilbene production of leaves (induced by mucic acid) and that of berries (induced by sac-charose solution) of 6 different grapevines were positively correlated. A negative correlation was found between stilbene production and susceptibility to B. cinerea. Einleitung