Where gene discovery turns into systems biology: genome-scale RNAi screens in Drosophila

Ralph A. Neumüller, Norbert Perrimon
2010 Wiley Interdisciplinary Reviews: Systems Biology and Medicine  
Systems biology aims to describe the complex interplays between cellular building blocks which, in their concurrence, give rise to the emergent properties observed in cellular behaviors and responses. This approach tries to determine the molecular players and the architectural principles of their interactions within the genetic networks that control certain biological processes. Large-scale lossof-function screens, applicable in various different model systems, have begun to systematically
more » ... rogate entire genomes to identify the genes that contribute to a certain cellular response. In particular, RNA interference (RNAi)-based highthroughput screens have been instrumental in determining the composition of regulatory systems and paired with integrative data analyses have begun to delineate the genetic networks that control cell biological and developmental processes. Through the creation of tools for both, in vitro and in vivo genomewide RNAi screens, Drosophila melanogaster has emerged as one of the key model organisms in systems biology research and over the last years has massively contributed to and hence shaped this discipline. 
doi:10.1002/wsbm.127 pmid:21197652 pmcid:PMC3560415 fatcat:n7pqximbwnatvnvtetd53u52fa