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Second Site Suppressor Mutations of a GTPase-deficient G-Protein α-Subunit
1998
Journal of Biological Chemistry
G proteins transmit signals from cell surface receptors to intracellular effectors. The intensity of the signal is governed by the rates of GTP binding (leading to subunit dissociation) and hydrolysis. Mutants that cannot hydrolyze GTP (e.g. G s ␣ Q227L , G i2 ␣ Q205L ) are constitutively activated and can lead to cell transformation and cancer. Here we have used a genetic screen to identify intragenic suppressors of a GTPase-deficient form of the G␣ in yeast, Gpa1 Q323L . Sequencing revealed
doi:10.1074/jbc.273.44.28597
pmid:9786851
fatcat:plarllj3tzeslollolzcafyi4a