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Improved Sensitivity and Resolution in the Flow Cytometric DNA Analysis of Human Solid Tumor SpecimensUse ofIn VitroFine-Needle Aspiration and Uniform Staining Reagents
1991
American Journal of Clinical Pathology
Twenty-five unselected fresh colon or breast tumors were studied to identify specific components of sample preparation, sample staining, and flow cytometer operation affecting the sensitivity of DNA stemline analysis. Solid tumors were disaggregated using both a published method for mechanical/enzymatic whole cell (M/EWC) isolation and a fine-needle aspiration (FNA) technique. Staining FNA samples with CycleTEST™ propidium iodide reagents demonstrated improved sensitivity in the recognition of
doi:10.1093/ajcp/96.5.615
fatcat:btkl3s3wobfodefzkaupqafoou