Effects of Seungseup-tang on MIA-induced Osteoarthritis Rats
승습탕(勝濕湯)이 MIA로 유발된 골관절염 흰쥐에 미치는 영향

Jong-Hoon Lee, Chang-Hoon Woo, Young-Jun Kim, Hee-Duk Ahn
2016 Journal of Korean Medicine Rehabilitation  
Objectives This study was designed to evaluate the anti-inflammatory effects of Seungseup-tang (SST) on the monosodium iodoacetate (MIA)-induced osteoarthritis rats. Methods Osteoarthritis was induced by injection of MIA (50 μl with 80 mg/ml) into knee joint cavity of rats. Rats were divided into 4 groups (normal group, control group, indomethacin treated group, SST treated group, each n=6). Normal group was not injected with MIA and taken normal diet. Control group was injected with MIA and
more » ... ted with MIA and taken with distilled water. Indomethacin treated group was injected with MIA and taken indomethacin 5 mg/kg by oral administration. SST treated group was injected with MIA and taken SST 200 mg/kg by oral administration. We examined the weight-bearing ability of hind paw, biomarkers related to oxidative stress in serum, inflammatory proteins and inflammatory mediators and cytokines. Moreover, histopathological examination of knee joint structure was also performed by Hematoxylin & Eosin (H&E), Safranin-O staining method. Results In the present study, SST treated group showed a similar inhibitory effects alike indomethacin treated group, in most of the studied parameters of inflammation. The increased oxidative stress biomarker such as reactive oxygen species (ROS) and peroxy nitrite (ONOO − ) in the serum were reduced with SST. Especially, the level of ONOO − compared with control group significantly suppressed. Also, the expression of inflammatory mediators and cytokines induced by nuclear factor-kappa B (NF-κB) activation was modulated through inhibition of IkBaphosphorlation. In addition, histological analysis revealed that cartilage damage by MIA repaired markedly in SST treated group. Conclusions According to the results, Seungseup-tang may be effective for preventing the progression of osteoarthritis. (J Korean Med Rehabil 2016;26(3):1-15) Key words Seungseup-tang, Osteoarthritis, Anti-inflammative effect, Monosodium iodoacetate (MIA) 서론»»» 골관절염(osteoarthritis, OA)은 퇴행성관절염이라고도 불리며, 성인에서 가장 흔한 관절 질환이다. 주로 체중부 하 관절을 많이 침범하여 관절 연골의 국소적인 퇴행성 변화, 연골하골의 비대, 주변 골연골부의 과잉 골형성, 관 절의 변형 등을 특징으로 하며 염증변화는 소수에서 나타 나고 임상적으로는 반복적인 동통, 관절 강직감 및 점진 적인 운동 장애 등을 초래한다 1) . 골관절염 발생의 주요 위험인자는 나이와 성별이며, 과 체중이나 반복적인 스트레스가 가해지는 직업, 선천적 형 태이상과 염증성 질환도 위험인자이다. 현대에서는 노령 이종훈⋅우창훈⋅김영준⋅안희덕 2 J Korean Med Rehabil 2016;26(3):1-15 인구의 증가에 따라 그 유병률도 증가하는 추세이며 55세
doi:10.18325/jkmr.2016.26.3.1 fatcat:rcfjhhp2pbdpznc5qws24qqly4