Control of vegf Expression and Tumor Angiogenesis by Nanoparticle-Berberine-Sanazole Complexes

S Sreeja, Krishnan Nair
2015 Human Journals Research Article November   unpublished
Growth of tumor is fully dependent on angiogenesis for the supply of nutrients which is essential for its proliferation and metastasis. Of the various pro-angiogenic factors,Vascular Endothelial Growth Factor (VEGF) plays a crucial role in the process. Tumor-induced angiogenesis was the key therapeutic target in the present study. The iron oxide nanoparticle-berberine-sanazole (NP-BBN-SAN) complexes were used to control the transcriptional level expression of vegf and thereby prevent
more » ... prevent neovascularization in animals bearing tumor in the peritoneal cavity. Results: The administration of NP-BBN-SAN complexes inhibited angiogenesis in mice-bearing tumor in the peritoneal cavity. The images of inner peritoneal membrane presented visual differences of angiogenesis in the animals treated with the complexes. At the transcription level the expression of the gene, vegf was found significantly down regulated in the tumor cells following the treatment with NP-BBN-SAN. The order of down regulation was NP-BBN-SAN> BBN> SAN. The complexes did not cause systemic toxicity to kidney and liver in these animals as evidenced from the results on serum biochemical parameters. Conclusion: The present work provide compelling evidence that nanoparticle-berberine-sanazole complexes down regulate vegf expression and thereby prevent neovascularization which is a must for tumor growth and metastasis. Thus the study reveals the feasibility of using the NP-BBN-SAN complexes in tumor therapy.