Protection ofpsbAIITranscript from Ribonuclease Degradationin Vitroby DnaK2 and DnaJ2 Chaperones of the CyanobacteriumSynechococcus elongatusPCC 7942

Satoru WATANABE, Masumi SATO, Kaori NIMURA-MATSUNE, Taku CHIBAZAKURA, Hirofumi YOSHIKAWA
2007 Bioscience, biotechnology and biochemistry  
Three dnaK and four dnaJ genes have been identified in the genome of cyanobacterium Synechococcus elongatus PCC 7942. Our comprehensive analysis of yeast two-hybrid screening revealed a specific interaction among DnaK2, DnaJ2, and RNase E, an essential endoribonuclease. We examined the effects of DnaK2 and DnaJ2 on RNase E activity by monitoring the digestion of psbAII transcript in vitro. The addition of DnaK2 and DnaJ2 obviously inhibited RNase E activity in an ATPdependent manner. These
more » ... manner. These results suggest that DnaK2 and DnaJ2 are involved in RNA degradation through interaction with RNase E.
doi:10.1271/bbb.60647 pmid:17213638 fatcat:uuj5nvo2krgt3opnprnqfam3me