Mitochondrial localization ofArabidopsis thalianaIsu Fe-S scaffold proteins

Sébastien Léon, Brigitte Touraine, Jean-François Briat, Stéphane Lobréaux
2005 FEBS Letters  
Isu are scaffold proteins involved in iron-sulfur cluster biogenesis and playing a key role in yeast mitochondria and Escherichia coli. In this work, we have characterized the Arabidopsis thaliana Isu gene family. AtIsu1,2,3 genes encode polypeptides closely related to their bacterial and eukaryotic counterparts. AtIsu expression in a Saccharomyces cerevisiae Disu1Dnfu1 thermosensitive mutant led to the growth restoration of this strain at 37°C. Using Isu-GFP fusions expressed in leaf
more » ... s and immunodetection in organelle extracts, we have shown that Arabidopsis Isu proteins are located only into mitochondria, supporting the existence of an Isu-independent Fe-S assembly machinery in plant plastids.
doi:10.1016/j.febslet.2005.02.038 pmid:15792798 fatcat:e7afclzx7zachho6sdlerl5gtq