Sustained activation of AMP-activated protein kinase induces c-Jun N-terminal kinase activation and apoptosis in liver cells

Delphine Meisse, Mark Van de Casteele, Christophe Beauloye, Isabelle Hainault, Benjamin A Kefas, Mark H Rider, Fabienne Foufelle, Louis Hue
2002 FEBS Letters  
The aim of this work was to study the e¡ect of a sustained activation of AMP-activated protein kinase (AMPK) on liver cell survival. AMPK activation was achieved by incubating FTO2B cells with AICA-riboside, which is transformed into ZMP, an AMP analogue, or by adenoviral transfection of hepatocytes with a constitutively active form of AMPK. Prolonged AMPK activation triggered apoptosis and activated c-Jun N-terminal kinase (JNK) and caspase-3. Experiments with iodotubercidin, dicoumarol and
more » ... AD-fmk, which inhibited AMPK, JNK and caspase activation, respectively, supported the notion that prolonged AMPK activation in liver cells induces apoptosis through an activation pathway that involves JNK and caspase-3. ß
doi:10.1016/s0014-5793(02)03110-1 pmid:12208500 fatcat:v7ztkc5m3nftpbomqc3366ovka