NMR of carotenoids - new experimental techniques

G. Englert
1985 Pure and Applied Chemistry  
A number of novel experimental one-and two-dimensional NMR techniques are presented which have been found to be extremely useful for the elucidation of the structures of carotenoids. These are the rotating-frame experiments TOCSY (Total Correlation Spectroscopy, also named HOHAHA) and ROESY (Rotating Frame Nuclear Overhauser Effect Spectroscopy). Moreover, some applications of the very sensitive 1H-detected one-bond and multiple-bond versions of 1H,13C-correlated 2D COSY will be presented.
more » ... be presented. These techniques will be applied to the derivation of the structures of the novel C40 retro carotenoid 1 ((3S,5R,3'S,5'R)-4,5-dihydro-4,5'-retro-f3,G-carotene-3,5,3',5'-tetrol) and of the hepta-and octaacetate derivatives & I and & of the microalgal glycosidic carotenoid P457 2 (13'-cis-7',8'-dihydroneoxanthin-2O-al 3'43-lactoside). Advantages and limitations of these techniques are discussed with respect to structure elucidations in the field of carotenoids.
doi:10.1351/pac198557050801 fatcat:udyrtahiardbzngsectnlgiy4e