Acceptability criteria for linear dependence in validating UV-spectrophotometric methods of quantitative determination in forensic and toxicological analysis

L. Yu. Klimenko, G. P. Petyunin, S. M. Trut, V. P. Moroz
2014 Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki  
Introduction. This article is the result of authors' research in the field of development of the approaches to validation of quantitative determination methods for purposes of forensic and toxicological analysis and devoted to the problem of acceptability criteria formation for validation parameter «linearity/calibration model». The aim of research. The purpose of this paper is to analyse the present approaches to acceptability estimation of the calibration model chosen for method description
more » ... ethod description according to the requirements of the international guidances, to form the own approaches to acceptability estimation of the linear dependence when carrying out the validation of UV-spectrophotometric methods of quantitative determination for forensic and toxicological analysis. Materials and methods. UV-spectrophotometric method of doxylamine quantitative determination in blood. Results. The approaches to acceptability estimation of calibration models when carrying out the validation of bioanalytical methods is stated in international papers, namely «Guidance for Industry: Bioanalytical method validation» (U.S. FDA, 2001), «Standard Practices for Method Validation in Forensic Toxicology» (SWGTOX, 2012), «Guidance for the Validation of Analytical Methodology and Calibration of Equipment used for Testing of Illicit Drugs in Seized Materials and Biological Specimens» (UNODC, 2009) and «Guideline on validation of bioanalytical methods» (ЕМА, 2011) have been analysed. It has been suggested to be guided by domestic developments in the field of validation of analysis methods for medicines and, particularly, by the approaches to validation methods in the variant of the calibration curve method for forming the acceptability criteria of the obtained linear dependences when carrying out the validation of UV-spectrophotometric methods of quantitative determination for forensic and toxicological analysis. The choice of the method of calibration curve is dictated by the primary orientation of all studied international guidances on t [...]
doi:10.14739/2409-2932.2014.2.26124 doaj:23e8a918fb864993b16cf14346620ff5 fatcat:7xe4vqjlrnajfgirhcmp2pzvui