Escherichia coli identification and strain discrimination using nanosecond laser-induced breakdown spectroscopy

Jonathan Diedrich, Steven J. Rehse, Sunil Palchaudhuri
2007 Applied Physics Letters  
Rehse, Steven J.; and Palchaudhuri, S.. (2007) . Escherichia coli identification and strain discrimination using nanosecond laser-induced breakdown spectroscopy. Applied Physics Letters, 90 (16). Three strains of Escherichia coli, one strain of environmental mold, and one strain of Candida albicans yeast have been analyzed by laser-induced breakdown spectroscopy using nanosecond laser pulses. All microorganisms were analyzed while still alive and with no sample preparation. Nineteen atomic and
more » ... ineteen atomic and ionic emission lines have been identified in the spectrum, which is dominated by calcium, magnesium, and sodium. A discriminant function analysis has been used to discriminate between the biotypes and E. coli strains. This analysis showed efficient discrimination between laser-induced breakdown spectroscopy spectra from different strains of a single bacteria species.
doi:10.1063/1.2723659 fatcat:cbshtpxty5huhebpsor42gx2hm