DNA FINGERPRINTING OF SINGLE APHID EMBRYOS BY RANDOM AMPLIFIED POLYMORPHIC DNA POLYMERASE CHAIN REACTION (RAPD–PCR)

C.K. Chan, D.J. Petersen, T.C. Vrain
1999 Canadian Entomologist  
Extraction of DNA from whole aphids, in combination with random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) (Williams et al. 1990) markers can detect interspecific and intraspecific genetic variation (Black et al. 1992; Cenis et al. 1993). However, these techniques entail destructive sampling of fresh or preserved specimens. To allow experimental replication from a single sample while preserving the same aphid for morphometrical or karyotyping analyses, we describe a
more » ... describe a technique for RAPD-PCR using DNA from single aphid embryos. We evaluated the usefulness and reliability of single-embryo analysis, using four species of our laboratory colonies, namelyAcyrthosiphon pisum(Harris),Aphis fabaeScopoli,Aphis frangulaegroup, andAphis gossypiiGlover.
doi:10.4039/ent131229-2 fatcat:vs3yzcmmrncvngwffpjvvfzylq