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Primerize: automated primer assembly for transcribing non-coding RNA domains
2015
Nucleic Acids Research
Customized RNA synthesis is in demand for biological and biotechnological research. While chemical synthesis and gel or chromatographic purification of RNA is costly and difficult for sequences longer than tens of nucleotides, a pipeline of primer assembly of DNA templates, in vitro transcription by T7 RNA polymerase and kit-based purification provides a cost-effective and fast alternative for preparing RNA molecules. Nevertheless, designing template primers that optimize cost and avoid
doi:10.1093/nar/gkv538
pmid:25999345
pmcid:PMC4489279
fatcat:6q7gcdga6vei7g7u6ix7i2mf6y