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The reproduction of the shape of giant vesicles usually results in the increase of their "population" size. This may be achieved on giant vesicles by appropriately supplying "mother" vesicles with membranogenic amphiphiles. The next "generation" of "daughter" vesicles obtained from this "feeding" is inherently difficult to distinguish from the original mothers. Here we report on a method for the consecutive feeding with different fatty acids that each provoke membrane growth and detachment ofdoi:10.1016/j.isci.2020.101677 pmid:33163935 pmcid:PMC7609504 fatcat:vwfnmvrrhjglbhzlnvszkt4rji