Extraction and separation of Antioxidative Compounds from Salvia leriifolia Leaves

R Farhoosh, H Purazrang, M Khodaparast, M Rahimizadeh, S Seyedi
2004 J. Agric. Sci. Technol   unpublished
In this research, methanolic extraction of Salvia leriifolia leaves produced a higher yield and antioxidative activity than other organic solvents (ethanol, acetone, chloroform, n-hexane). The methanolic extract was reserved about 24 hours in the refrigerator and its precipitates were then separated. The extract was separated into 12 fractions by thin-layer chromatography (TLC). The highest yields were found in fractions with R f values of 0.29, 0.54, 0.11 and 0.38 at 16.24%, 12.48%, 8.81% and
more » ... .60%, respectively. All fractions and also whole methanolic extract and precipitates of methanolic extract had more anti-oxidative activity than the control based on the thiocyanate method. Whole methanolic extract , precipitates of methanolic extract and most separated fractions showed more anti-oxidative activity than α-tocopherol. The fraction with R f value of 0.29 at 16.24% yield and 85.61% antioxidative activity of synthetic antioxidant BHT based on thiocyanat method was chosen as the fraction with the highest yield and the highest antioxidative activity .