This experiment was designed to investigate the roles of induced pluripotent stem cells and hormonal therapy on experimentally-induced ovarian ablation (OA) by evaluating of hormonal (E2, FSH, LH), molecular (Oct4, Runx2, Stra8), histopathological (H&E) and immunohistochemical (PCNA) examinations. Fifty female rats were classified into five main groups. Group (I): 10 rats as control normal group. Group II (OA):10 rats infused with (3 mg/kg) of DOX and (50 mg) CYP dissolved in clean

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... saline I/P once a week for 5 weeks. Group (III):10 rats go through chemoablation, after that, rats were injected with 5 IU of PMSG single subcutaneous (S/C) injection. Group (IV): 10 rats undergo chemoablation, after that induced pluripotent stem cells (iPSc) were injected intravenous (I/V) single injection for 2 months. Group (V): 10 rats undergo chemoablation then iPSc were injected I/V combined with single S/C injection of 5 IU PMSG. Results revealed that rats injected with doxorubicin and cyclophosphamide showed a significant increase in FSH, LH levels while a significant decrease in E2 level, PCNA, Oct4,Stra8 and Runx2 when compared with control (-ve) group. On the other hand, rats treated with PMSG or IPSc only or PMSG and IPSc revealed that there were increase inE2 level, PCNA, OCT4, Stra8 and Runx2 while there is decrease in FSH, LH levels when compared with their chemo-ablated control group. Also, ovarian sections of chemo-ablated group showed degenerated surface epithelia, loss of secondary and antral follicles as well as degenerated cortex and medulla. PMSG treated group revealing development of follicles in normal sequence, but in rudimentary number. Also, group was treated with iPSc revealing ovary with normal architecture harboring a high number of anular follicles. In contrast ovarian sections of group treated with PMSG and iPSc showed well observed primordial, primary, and secondary follicles.