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Extraction is an important procedure for samples that contain soil because other compounds in soil may affect analysis of estrogens. This study was conducted to evaluate three different extraction methods for 17β-estradiol in soil. Sand, bentonite, and organicrich silt loam were spiked with 1 mg kg −1 of 17β-estradiol as a model compound of estrogens. 17β-estradiol and its metabolites, estrone and estriol, were extracted using (i) a modified Bligh and Dyer extraction, (ii) a pressurized fluiddoi:10.1080/03601230500189006 pmid:16190017 fatcat:golifamhgjaijd5focm3xvcssm