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This study is to establish fibroblast line of AA broiler chicken from frozen embryos. According to the requirement of (ATCC) tests, the study depended on testing several points such as growth curve, cryopreservation, Karyotyping of chromosomes and protein translation of transfected vector. The results showed that the cells were morphologically consistent with fibroblasts and the growth curve was sigmoidal with a population doubling time (PDT) was(41.6h FE , 43.2h AFE). Cell viability was 91.8%fatcat:ymchv3uxovch7lahzxwvmf7ge4