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A Comparison of Deep Sequencing of TCRG Rearrangements vs Traditional Capillary Electrophoresis for Assessment of Clonality in T-Cell Lymphoproliferative Disorders
2014
American Journal of Clinical Pathology
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Objectives: To design and evaluate a next-generation sequencing (NGS)-based method for T-cell receptor g (TCRG) gene-based T-cell clonality testing on the Ion Torrent Personal Genome Machine (Life Technologies, Carlsbad, CA) platform. Methods: We analyzed a series of peripheral blood, bone marrow, and formalin-fixed paraffin-embedded tissue specimens with NGS vs traditional capillary electrophoresis methods. Results: Using a custom analysis algorithm that we developed, our NGS assay identified between 2, 215 and 48,222 unique
doi:10.1309/ajcp5tygbvw4zitr
pmid:24515762
fatcat:y4lsjfnwizgvvknxtzwipjnhuu