MORPHOLOGIC CHANGES OF THE BRAIN TISSUE AFTER THE ADMINISTRATION OF IONIZED LIQUIDS WITH DIFFERENT OXIDATION-REDUCTION POTENTIAL DURING ANAPHYLACTIC SHOCK AND BRAIN ISCHEMIA

V.I. Kharina I.V. Kovalenko
2019 Zenodo  
The purpose of the study: to study the impact of anolyte and catholyte on the structural changes of the brain tissue after their intravenous administration during anaphylactic shock and brain ischemia in laboratory animals. Methodology: anaphylactic shock was modelled at 40 laboratory rabbits by administration of horse serum of blood after 21-day sensitization of the animals. The test liquids (0.9% saline solution, prednisolone, anolyte, catholyte) were administered 20 minutes before the
more » ... s before the administration of the recall dose correspondingly to each of the 4 groups. Brain sampling for the morphologic analysis was conducted on the 7th day of the study. Experimental brain ischemia was modelled at 33 laboratory rabbits surgically - by the right common carotid (RCC) artery ligation. The agents under study (water for injections, anolyte, catholyte) were administered daily for 14 days correspondingly to each of the 3 groups. Sampling for the study was conducted on the 14th day of the experiment. The findings of the study showed the inexpediency of the catholyte intravenous use during anaphylactic shock. The anolyte intravenous use during anaphylactic shock does not affect adversely on the brain tissue. The morphologic analysis of the laboratory rabbits' brain tissue detected the reduction of adverse effects, which points at the antiallergic action of the test liquid. The water for injections and anolyte administration does not affect the building-up processes of severe destructive changes of the neurocytes of the brain cerebral hemispheres frontal cortex and hippocampus, having irreversible character, appearing after experimental brain ischemia (EBI) (the right common carotid artery ligation). According to the morphologic data, the adverse therapeutic outcome is possible after the administration of liquids with a big positive ORP (oxidation reduction potential) to the ischemic animals, which makes their use during BI (brain ischemia) inexpedient. It is found that the minimum brain tissue damage after ischemia out of all [...]
doi:10.5281/zenodo.3471668 fatcat:3n3sjbxmmrbi3kbh3e7jmdqt54