Improvement of infarcted heart by the transplantation of adipose-derived stem cells with injectable temperature-responsive chitosan hydrogel

J. Yang, Z. Liu, Z. Yuan, Y. Chen, C. Wang
2011 Heart  
Objective Stem cell-based therapy can preserve cardiac function but is limited by the low cell retention within ischemic tissues. Injectable tissue engineering materials are well known for maintain cell survival and enhance cell-based therapies' effi cacy. We aimed to studying the usefulness of the combination of Injectable temperature-responsive chitosan hydrogel (as scaffold) and adipose-derived stem cells (ADSCs) to treat myocardial infarction. Methods Rat ADSCs were isolated from
more » ... s adipose tissues and further confi rmed by its surface phenotype as well as multi-potential differentiation. Temperature-responsive chitosan hydrogel (chitosan), made by specifi c technique, was then co-injected with ADSCs into the left ventricular wall of rat myocardial infarction models. The structure and functional consequences of transplantation were determined by detailed histological analysis and echocardiography.
doi:10.1136/heartjnl-2011-300867.35 fatcat:yumvfpxuirh3nohe7j3vjgwmqi