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Single Molecule Unzipping of Coiled Coils: Sequence Resolved Stability Profiles
2006
Physical Review Letters
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We use a high resolution atomic force microscopy technique to mechanically unzip and rezip single coiled-coil proteins. This allows us to read off the complete stability profile of the protein turn by turn. We investigated three coiled coils with different length as well as a point mutation and find force fluctuations between 9 and 15 pN that can be directly related to the amino-acid sequences. An equilibrium model previously applied to DNA fully describes the mechanical unzipping process
doi:10.1103/physrevlett.96.118102
pmid:16605876
fatcat:76o4gnmrr5gmhhys7pi6qgdsqy