Single Molecule Unzipping of Coiled Coils: Sequence Resolved Stability Profiles

Thomas Bornschlögl, Matthias Rief
2006 Physical Review Letters  
We use a high resolution atomic force microscopy technique to mechanically unzip and rezip single coiled-coil proteins. This allows us to read off the complete stability profile of the protein turn by turn. We investigated three coiled coils with different length as well as a point mutation and find force fluctuations between 9 and 15 pN that can be directly related to the amino-acid sequences. An equilibrium model previously applied to DNA fully describes the mechanical unzipping process
more » ... ing free-energy contributions of the individual turns and seed formation energy.
doi:10.1103/physrevlett.96.118102 pmid:16605876 fatcat:76o4gnmrr5gmhhys7pi6qgdsqy