Analysis of intra-population variability of bald cypress (Taxodium distichum L. Rich.) in seed stand near Backa Palanka using RAPD markers

Vladan Popovic, Aleksandar Lucic, Danijela Ristic, Ljubinko Rakonjac, Sabahudin Hadrovic, Snezana Mladenovic-Drinic
2015 Genetika  
Drinić (2015) : Analysis of intra-population variability of bald cypress (Taxodium distichum L. rich.) in seed stand near Vol 47, No. 2,[571][572][573][574][575][576][577][578][579][580] The analysis of Bald cypress genetic variability at the level of test trees was performed using RAPD (Random Amlified Polymorphic DNA) markers. RAPD analysis was performed on 20 test trees with 13 primers. A total of ten primers gave a clear picture while three primers amplified weakly. 60 is a total number of
more » ... etected bands obtained by RAPD analysis with 10 selected primers, and the average number of bands is 6. Based on presence/absence of RAPD fragments among all 20 Bald cypress test trees were calculated similarity coefficients by Dice and they range from 0.73 to 1. Based on similarity coefficients was performed the cluster analysis and results were presented as a dendrogram. All 20 test trees were grouped into two sub-clusters. Test trees 1, 4 and 11 were grouped in the first sub-cluster while other test trees were grouped in the second sub-cluster. By analysis of relations within every sub-cluster and sub-sub-cluster the existence of genetic distances between observed test trees can be noticed. The greatest similarity is between test trees 2, 12, 15 and 18. The results of genetic similarity and distance between observed test trees indicate the overwhelming presence of genetic diversity.
doi:10.2298/gensr1502571p fatcat:mdljjpp6abdhli3iprbos7koji