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Immunoelectron microscopic observation of β-lactoglobulin in paneer cheese using the Tokuyasu method
Immunoelectron microscopy is a useful electron microscopy observation method for dairy product analysis to determine the localization of speciˆc proteins. We conˆrmed the usefulness of the Tokuyasu method (frozen ultrathin section method) by visualizing the distribution of b-lactoglobulin in a paneer cheese sample obtained by acid coagulation after heating raw bovine milk. In the control test (post-embedding method), as the protein was ˆxed only with paraformaldehyde, the milk proteindoi:10.11465/milk.68.94 fatcat:4e3qzspkufgu7hwf6o34ojghmq